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Can Anchoring Fibrils be reinforced by nanofibers?

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Anchoring fibrils are polymers of Type VII collagen that connects basement membrane of the skin to the dermal connective tissue. Abnormalities of anchoring fibrils lead to a group of disparate and debilitating disorders called dystrophic epidermolysis bullosa (DEB). The mutation analyses and studies on genotype-phenotype correlations in epidermolysis bullosa have revealed an unusual complexity of the gene defects and their biological consequences. I even tried machine learning techniques to make sense of this complex genotype - phenotype correlation in the simplex variant.

The article below demonstrates the use of nanofibers to stabilise anchorage-dependant animal cells. The current emphasis is on finding a gene therapy treatment approach for this condition. May be a biophysical approach as below can also be tried!

"Biotechnol Lett. 2011 Dec 30. 

Efficient formation of cell spheroids using polymer nanofibers.

Shin JY, Park J, Jang HK, Lee TJ, La WG, Bhang SH, Kwon IK, Kwon OH, Kim BS.

Spheroid culture has been used for suspension cultures of anchorage-dependent
cells. In this study, we developed a new method for the suspension cultures of
anchorage-dependent animal cells using polymer nanofibers.
Poly(lactic-co-glycolic acid) nanofibers (785 nm in average fiber-diameter, 88 μm
in average fiber-length) fabricated by the electrospinning method were added to
each suspension culture of human embryonic kidney 293 cells and human dermal

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About Me

As a Dermatologist and Informatician my research mainly involves application of bioinformatics techniques and tools in dermatological conditions. However my research interests are varied and I have publications in areas ranging from artificial intelligence, sequence analysis, systems biology, ontology development, microarray analysis, immunology, computational biology and clinical dermatology. I am also interested in eHealth, Health Informatics and Health Policy.


Bell Raj Eapen
Hamilton, ON