Anchoring fibrils are polymers of Type VII collagen
that connects basement membrane of the skin to the dermal connective tissue. Abnormalities of anchoring fibrils
lead to a group of disparate and debilitating disorders called dystrophic epidermolysis bullosa
(DEB). The mutation analyses and studies on genotype-phenotype correlations in epidermolysis bullosa have revealed an unusual complexity of the gene defects and their biological consequences. I even tried machine learning techniques
to make sense of this complex genotype - phenotype correlation in the simplex variant.
"Biotechnol Lett. 2011 Dec 30.
The article below demonstrates the use of nanofibers
to stabilise anchorage-dependant animal cells. The current emphasis is on finding a gene therapy treatment approach for this condition. May be a biophysical approach as below can also be tried!
Efficient formation of cell spheroids using polymer nanofibers.
Shin JY, Park J, Jang HK, Lee TJ, La WG, Bhang SH, Kwon IK, Kwon OH, Kim BS.
Spheroid culture has been used for suspension cultures of anchorage-dependent
'via Blog this'
cells. In this study, we developed a new method for the suspension cultures of
anchorage-dependent animal cells using polymer nanofibers.
Poly(lactic-co-glycolic acid) nanofibers (785 nm in average fiber-diameter, 88 μm
in average fiber-length) fabricated by the electrospinning method were added to
each suspension culture of human embryonic kidney 293 cells and human dermal
Labels: Genodermatosis, Nanofiber, research